Chioma Nwakanma has completed her Ph.D at the age of 30 years from University of Port Harcourt, Nigeria. She is a Lecturer in the College of Natural Resources and Environmental Management. She has published more than 17 papers in reputed journals and has Professional affiliations both local and international.
The impact of oil- based drilling mud disposal in Southern Nigeria was studied using static plastic tanks in the laboratory on Periopthalmus papilio. Th e Niger Delta is one of the world’s largest wetlands encompassing about 2000km2 in Southern Nigeria. Most of the petroleum exploration and production activities are located in the coastal zones where great numbers of aquatic organisms live like the mudskipper fi sh. Th e experiment was conducted in triplicate for each concentration. The histopathological study of the eff ects of exposure to a lethal concentration (2%, 1.6%, 0.8%, 0.4%, and 0%) of oil - based drilling mud on the amphibious fi sh indicated mild to severe damage to liver, kidney, muscle, intestine, brain and gills. Aft er a 14 days exposure, the percentage mortality against concentration was determined. The result revealed that the oil - based drilling mud were toxic to the tissues examined as no noticeable change were observed in tissues of the control (0%) tanks.
Ogbulie Toochukwu Ekwutosi has completed her Ph.D at the age of 34 years from Federal University of Technology Owerri (FUTO) Nigeria. She has also obtained additional qualifi cation as Registered Environmental Scientist by NREP, USA in 2008. She is a senior Lecturer in the Department of Biotechnology, FUTO, Nigeria. She has published more than 30 papers in reputed journals and has been serving as an Editorial Board Member of Analele Universitatii din Oradea - Fascicula Biologie, Romania. She is a member of societal bodies as Society for Applied Microbiology UK (SFAM), Graduate Women in Science GWIS USA, and Organization for Women in Science for the Developing World (OWSD).
This study looked at the diversity of microorganisms persistent in agricultural soil sample polluted with Nigerian Bonny light crude oil for four years with a view to ascertain the presence of microbes with probable degradative gene for crude oil degradation and to confi rm similarities in microbial identities. DNA from crude oil polluted agricultural soil sample was extraction using ZYMO soil DNA extraction Kit. DNA sequencing was performed thereaft er, by Next Generation Sequencing Technique (NGST) using automated PCR cycle- Genome Sequencer™ FLX System from 454 Life Sciences™ and Roche Applied. Sequence analysis and alignment was performed using Vecton NTI suite 9 and the resulting nucleotide sequences were compared to sequences obtained from GenBank by BLASTx analysis using CLO Bio soft ware as well as BLASTn using NCBI. Molecular confi rmation of similarities in microbial Identities was obtained by creating diff erent dendrograms/ distance trees. Gene sequencing carried out read 513 diff erent nucleotide sequences. Every read was BLASTED and the result fi le saved. Nine phylum with 47 corresponding culture-dependent species and 169 culture-independent bacteria clone was obtained. The resultant tree however, showed various proteobacteria (a-, b-, d- and g- proteobacteria), bacteria, enterobacteria, firmicutes, plantomycetes, acidobacteria group/fi brobacteres, Bacteriodetes/chlorobi Actinobacteria/high G+C and chlorifl exi phyla. The nucleotide sequences with no hit was sent to Genbank for asigning of ascension number. Th e isolation of the aforementioned organisms from crude oil polluted agricultural soil left for four years, depict that the organism probably, have degradative genes which aided their survival.