Biodiversity

Biography

Biography: Dwi Astuti

Abstract

Enggano Island has Psittacula parakeet bird; Psittacula longicauda modesta. Phylogenetically, the position of the bird has not been studied yet. The recent study used DNA sequences of mitochondrial ND2 gene to analyze phylogenetic relationships within Psittacula parakeet birds. Blood samples were collected from two Psittacula species; Psittacula alexandri from Jawa Island, and Psittacula longicauda from Enggano Island and Natuna Island. Blood samples were taken from each bird and DNA was extracted from each blood sample. PCR was performed to amplify single DNA fragment of ND2, cytochromeb (Cyt B) and COI genes, separately using a pair of nucleotide primer. The DNA targets were then sequenced. Totally, 1020-bp of Cyt B gene was used to calculate genetic divergence within and between Psittacula parakeet, and to construct phylogenetic trees. DNA sequence data of Psittacula longicauda and others Psittacula species were taken from GenBank. Columba livia, Accipiter, and Cacatua were used as out group species. The genetic divergences within Psittacula longicauda were 3.70 % for (P.l. modesta vs. P.l. defontainei), 2.50 % for (P.l. defontainei vs. P.l. longicauda), and 1.60 % for (P.l. modesta vs. P.l. longicauda). Phylogenetic trees (NJ and ML trees) showed that based Psittacula longicauda was monophyletic species. Psittacula parakeet from Enggano Island (P.l. modesta) was separated from P.l. longicauda (from Sumatra/Borneo Island) and P.l. defontainei (from Natuna Island).